Part:BBa_K3504023

Off Switch

NOTICE: Parts in our range for this season have been created as a part of our Phase I design of our project. These parts HAVE NOT been tested or characterized in the lab due to COVID-19-related precautionary measures. We have enriched our new parts pages with data from literature and results from our modeling and simulations. If you are intending on using this part or others in our range, please keep in mind these limitations and update these parts with data from your experimentation. Feel free to reach us at: igem.afcm@gmail.com for further inquiries.

Part Description

A composite of parts (BBa_I712004,BBa_K3504019,BBa_K3504008) Which form as a whole an off switch using mir-126 & FF4 Mirna (BBa_K3504012) as as an inputs for a NOR gate that could protect our replicon vaccine vectors from being attacked by innate immunity inside dendritic cells and act as a safety switch

Characterization

We characterized this part by structural modelling and simulation of interaction between ff4 and its binding site using ff4 as an input for a NOR gate that could protect our replicon vaccine vectors from being attacked by innate immunity inside dendritic cells and act as a safety switch

Figure 2.This figure shows the sbol format of this composite part as well as way in which a nor gate works.

Figure 1.These figures illustrate the RNA Fold structure of multiple MiRNAs comparing there stabilities by free energy thermodynamic and showing there ability to induce down regulation of the circuit and work as an off switch.

Figure 3.This figure illustrate the interaction between FF4 and its binding site that showed energy equal to -3.14kcal\mol.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]