Composite

Part:BBa_K3332046

Designed by: Shengyang Zhang   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 19:18, 17 October 2020 by Registry (Talk | contribs)

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J23100-RBS-his-tag-iNAP-terminator

This is a fusion protein of TEX and cpYFP that can be purified with the function of His-Tag on the N-terminal. We use it to construct a new part that can be purified with the function of his-tag on the N-terminal and detect NADPH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 351
    Illegal XhoI site found at 377
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 117
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None