Regulatory

Part:BBa_K3504017

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-10-15)
Revision as of 12:46, 22 October 2020 by AhmedAdel01 (Talk | contribs) (→‎Usage)


SP6 Promoter

Part Description

Sp6 is a constitutive promoter from Sp6 bacteriophage but requires SP6 RNA polymerase When used for in vitro transcription, and according to its orientation the promoter transcribes either the sense OR antisense transcript.

Usage

SP6 promoter controls the transcription of the nsPs genes of pSFV; therefore, in vitro transcription and capping of the 5′ end are required for the expression of the gene of interest in mammalian cells.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None