Part:BBa_K3338002
Synthetic promoter_2 with NF-κB and AP1 binding sites
Usage and Biology
The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-κB/AP1 pathway leading to strong induction of gene expression. Due to its relatively low basal activity and strong induction following LPS supplementation it is perfectly suitable for the application as a LPS-sensor. It was designed as an improvement of the CMV promoter making it LPS-sensing. This improvment is also documented on the wiki page of the original part BBa_I712004. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated AP1-NF-κB binding sites are localized.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
Illegal NotI site found at 58 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//chassis/eukaryote
//chassis/eukaryote/human
//function/sensor
//promoter
//regulation/positive
None |