Coding
iNAP

Part:BBa_K3332000

Designed by: Shuling Xiao   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 17:55, 17 October 2020 by Registry (Talk | contribs)

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iNAP-his-tag

It is a fusion protein of TEX and cpYFP that can be purified with the function of His-Tag on the N-terminal. We use it to construct a new part that can be purified with the function of His-Tag on the N-terminal and detect NADPH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 272
    Illegal XhoI site found at 298
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None