Part:BBa_I15010

cph8 (Cph1/EnvZ fusion)

Chimeric Cph1 light receptor/EnvZ protein.

Usage and Biology

Requires PCB biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation. The light responsive domain (Cph1) has maximal response to light near 660nm. Exposure to red light inhibits the activity of the EnvZ histidine kinase domain. When active (in the dark) EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpC promoter and represses transcription from the OmpF promoter. Must be used in E.coli deficient in natural EnvZ.

Some user-experiences and characterization experiments related to the bacterial photography system are documented here [http://openwetware.org/wiki/Talk:BE.109:Systems_engineering/RT-PCR_data_analysis]. In general, experiments were conducted by students in BE.109, spring 2006, and involved variation of the strain's growth conditions or light exposure. The consequences of each experimental variation were followed with b-gal assay and RT-PCR.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2257
  Illegal XhoI site found at 364
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

••••• University of Macau iGEM 2020

2020 team UM_Macau characterization Group: 2020 team UM_Macau Author: Leong Chan Tat Cph8 requires PCB gene for chromophore formation. Inhibit the autophosphorylation in the EnvZ and transfer phosphoryl to OmpR in a dark environment. UM_Macau 2020 employed Bba_I15010 as the receptor of PCB. And express the downstream composite part (BBa_K3630028). That we will use this system to finish a dark repression system.