Composite
Part:BBa_K3505026
Designed by: Asteria Tsapadikou ,Emmanouil Stylianakis Group: iGEM20_Thessaly (2020-10-20)
ParaBAD:RBS- β-arrestin2:TEVp:terminator
long
Usage and Biology
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector BBa_K3505009 and has overhangs compatible for Golden Braid cloning.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 2854
Illegal PstI site found at 1546
Illegal PstI site found at 1633 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2854
Illegal PstI site found at 1546
Illegal PstI site found at 1633 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1446
Illegal BamHI site found at 1148
Illegal XhoI site found at 1843
Illegal XhoI site found at 2237 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 2854
Illegal PstI site found at 1546
Illegal PstI site found at 1633 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2854
Illegal PstI site found at 1546
Illegal PstI site found at 1633
Illegal AgeI site found at 983
Illegal AgeI site found at 1829 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 965
Illegal SapI.rc site found at 2788
Illegal SapI.rc site found at 3136
[edit]
Categories
Parameters
None |