Part:BBa_K3463017:Experience

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Characterization BBa_K3463017

TTo validate the UTR designer prediction and check the B0017 relevance, we measured the fluorescence and the absorbance of the cultures of the strains containing one of the following constructs: PtacHigh-eGFP, PtacLow-eGFP, PtacHigh-eGFP-B0017 BBa_K3463018, PtacLow-eGFP-B0017 BBa_K3463017 , and a non-transformed strain as a negative control for 4,5h. The experiment was carried out 3 times.

We obtained the following result shown in figure 1.

Figure 1 Effect of UTR prediction and B0017 terminator on eGFP fluorescence expression. The Figure 5 shows that no fluorescence was detected in the culture of the control non- transformed strain. As predicted by UTR designer the strong 5’UTR sequence (high) gave higher fluorescent levels than the weak 5’UTR sequence (Low). The terminator B0017 seems to have a limited effect on the fluorescent levels. The last seems to only impact the eGFP production of the strong UTR sequence.

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