Coding
Part:BBa_K3384315
Designed by: Yiqing Zhang Group: iGEM20_NJTech_China (2020-10-26)
Ste5ΔN-CTM
The highly active pheromone-independent Ste5 mutant.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 365
Illegal SpeI site found at 1620
Illegal SpeI site found at 2166 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 365
Illegal SpeI site found at 1620
Illegal SpeI site found at 2166 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 365
Illegal BglII site found at 813
Illegal BglII site found at 2121
Illegal XhoI site found at 2058 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 365
Illegal SpeI site found at 1620
Illegal SpeI site found at 2166 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 365
Illegal SpeI site found at 1620
Illegal SpeI site found at 2166 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
chassis | Saccharomyces cerevisiae |