Collections/iGEM Type IIS Collections
Team Uppsala 2020's iGEM Type IIS Standard collection
Until very recently, the main assembly standard supported by the iGEM registry was the BioBrick standard RFC[10]. However, iGEM Type IIS RFC[1000] assembly was recently accepted as a registry standard, and multiple Type IIS backbones and parts were present in the 2019 DNA distribution kit. We strongly believe in the efficiency and power of this cloning method since it can assemble multiple parts in one reaction, and we wanted to contribute to the development of this standard so that it can be used comfortably by the iGEM community.
Our parts collection provides three (four?) types of Type IIS parts. Firstly, it includes the new backbones detailed in the previous section as well as an improved version of pEven Level 2 backbones. Then, it includes dummy parts which can be used as placeholders when your planned assembly has less than four parts, the amount needed for iGEM Type IIS reactions. Finally (Afterwards?), it includes template parts which have no biological function, but are instead easy to use blueprints to design parts for this assembly method. (Finally, it includes some general use (parts, such as) RBS, which are tailored specifically for iGEM Type IIS assembly).
Sequencing files of all the parts can be downloaded here.
Plasmid Backbones
| Name | Description | Type | Created by | length | uses | seq |
|---|---|---|---|---|---|---|
| BBa_K3425001 | pSB3C11: Improved pEven1 Loop Vector based on pSB3C01 | Plasmid_Backbone | Nria Garriga Alonso | 2729 | -1 | . . . cccttagtgactcgaattcggtctcaggag |
| BBa_K3425002 | pSB3C12: Improved pEven2 Loop Vector based on pSB3C02 | Plasmid_Backbone | Nria Garriga Alonso | 2729 | -1 | . . . cccttagtgactcgaattcggtctcatact |
| BBa_K3425003 | pSB3C13: Improved pEven3 Loop Vector based on pSB3C03 | Plasmid_Backbone | Nria Garriga Alonso | 2729 | -1 | . . . cccttagtgactcgaattcggtctcaaatg |
| BBa_K3425004 | pSB3C14: Improved pEven4 Loop Vector based on pSB3C04 | Plasmid_Backbone | Nria Garriga Alonso | 2729 | -1 | . . . cccttagtgactcgaattcggtctcagctt |
| BBa_K3425005 | pSB3K01: pOdd1 Loop Vector based on pSB3K3 | Plasmid_Backbone | Nria Garriga Alonso | 2739 | -1 | . . . aggatgatttctggaattcgctcttcaatg |
| BBa_K3425006 | pSB3K02: pOdd2 Loop Vector based on pSB3K3 | Plasmid_Backbone | Nria Garriga Alonso | 2739 | -1 | . . . aggatgatttctggaattcgctcttcagca |
| BBa_K3425008 | pSB3K04: pOdd4 Loop Vector based on pSB3K3 | Plasmid_Backbone | Nria Garriga Alonso | 2739 | -1 | . . . aggatgatttctggaattcgctcttcacag |
| BBa_K3425009 | pSB4K01: pOdd1 Loop Vector based on pSB4K5 | Plasmid_Backbone | Nria Garriga Alonso | 3444 | -1 | . . . aggatgatttctggaattcgctcttcaatg |
| BBa_K3425010 | pSB4K02: pOdd2 Loop Vector based on pSB4K5 | Plasmid_Backbone | Nria Garriga Alonso | 3444 | -1 | . . . aggatgatttctggaattcgctcttcagca |
| BBa_K3425011 | pSB4K03: pOdd3 Loop Vector based on pSB4K5 | Plasmid_Backbone | Nria Garriga Alonso | 3444 | -1 | . . . aggatgatttctggaattcgctcttcatac |
| BBa_K3425012 | pSB4K04: pOdd4 Loop Vector based on pSB4K5 | Plasmid_Backbone | Nria Garriga Alonso | 3444 | -1 | . . . aggatgatttctggaattcgctcttcacag |
| BBa_K3425013 | pSB4A01: pOdd1 Loop Vector based on pSB4A5 | Plasmid_Backbone | Nria Garriga Alonso | 3420 | -1 | . . . aggatgatttctggaattcgctcttcaatg |
| BBa_K3425014 | pSB4A02: pOdd2 Loop Vector based on pSB4A5 | Plasmid_Backbone | Nria Garriga Alonso | 3420 | -1 | . . . aggatgatttctggaattcgctcttcagca |
| BBa_K3425015 | pSB4A03: pOdd3 Loop Vector based on pSB4A5 | Plasmid_Backbone | Nria Garriga Alonso | 3420 | -1 | . . . aggatgatttctggaattcgctcttcatac |
| BBa_K3425016 | pSB4A04: pOdd4 Loop Vector based on pSB4A5 | Plasmid_Backbone | Nria Garriga Alonso | 3420 | -1 | . . . aggatgatttctggaattcgctcttcacag |
To include a part in this table, include the categories "//collections/typeiis/uppsala" and set the part type to Plasmid_Backbone under the Hard Information tab of the part.
Dummy parts
There are no parts for this table
RBS
| Name | Description | Type | Created by | length | uses | seq |
|---|---|---|---|---|---|---|
| BBa_K3425031 | RBS for Type IIS iGEM Standard Assembly | RBS | Tereza Hubackova | 18 | -1 | tcacacaggaaagtacta |
| BBa_K3425032 | RBS for Type IIS iGEM Standard Assembly | RBS | Tereza Hubackova | 17 | -1 | aaagaggagaaatagta |
| BBa_K3425033 | RBS for Type IIS iGEM Standard Assembly | RBS | Tereza Hubackova | 20 | -1 | attaaagaggagaaatagta |
To include a part in this table, include the categories "//collections/typeiis/uppsala" and set the part type to RBS under the Hard Information tab of the part.