Part:BBa_K3606100

J23106-RBS-luxI-B0015-J23106-RBS-luxR

J23106-RBS-luxI-B0015-J23106-RBS-luxR is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project.

Usage and Biology

The promoter luxpR is activated by a complex comprised of a small molecule called AHL(acyl-homoserine lactone) and a constitutively expressed protein luxR which can bind AHL. LuxI encodes a synthase that converts SAM into AHL. When the population of our bacteria is low,the amount of the complex is not enough, thus the luxpR promoter cannot initiate expression of downstream genes. When the population of bacteria reaches a certain level, the AHL-luxR complex triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption. See more information about luxpR-CaAP on BBa_K3606044.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 701
  Illegal EcoRI site found at 1074
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1074
  Illegal NheI site found at 11
  Illegal NheI site found at 34
  Illegal NheI site found at 1096
  Illegal NheI site found at 1119
  Illegal SpeI site found at 702
  Illegal PstI site found at 716
  Illegal NotI site found at 709
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1074
  Illegal BglII site found at 694
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 702
  Illegal EcoRI site found at 1074
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1074
  Illegal SpeI site found at 702
  Illegal PstI site found at 716
  
• 1000
  COMPATIBLE WITH RFC[1000]