Regulatory

Part:BBa_K3527000

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_HS   (2020-10-17)
Revision as of 13:39, 26 October 2020 by Zhuke18 (Talk | contribs)


PhoA Promoter

PhoA Promoter


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Contribution

The phoA promoter is repressed by high phosphate concentrations. Under phosphate limited conditions, phoA promoter (basic parts BBa_K3527000) is activated. There are the other two phoA promoter sequence (basic parts: (BBa_K1139200 and BBa_K737023) which are much shorter than that of BBa_K3527000, as indicated by the following figure 1.

Figure 1

Engineering Success

Regulation of phoA promoter by phosphate concentration The function of phoA promoter was evaluated by the regulation of transcription of the gene of enhanced green fluorescent protein (EGFP). Using this reporting system, we showed that compared with the control, the bacteria containing PhoA-EGFP showed a significant fluorescence intensity difference (Figure 1) in the absence of phosphate in the medium. When the phosphate concentration added into the culture, the fluorescence intensity decreased to a level similar to the control culture.

Figure 2
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Categories
Parameters
None