Composite

Part:BBa_K3453114

Designed by: Maeva Cherriere   Group: iGEM20_Evry_Paris-Saclay   (2020-10-04)
Revision as of 13:02, 4 October 2020 by Maeva (Talk | contribs)


DmMatK Toehold Switch 2.1 with sfGFP-LVAtag

This part is an sfGFP-LVAtag (BBa_K2675006) expression cassette under the control of the DmMatK Toehold Switch 2.1 (BBa K3453014) for sequence-based detection of the rosewood species Dalbergia maritima var. pubescens.

Usage and Biology

In this composite part sfGFP-LVAtag (BBa_K2675006) was equipped by the synthetic DmMatK Toehold Switch 2.1 (BBa K3453014) and placed under the control of the T7 promoter (BBa_K2150031) and of the strong SBa_000587 synthetic terminator (BBa_K3453000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 38
    Illegal BamHI site found at 79
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 141
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None