Composite

Part:BBa_K3378001

Designed by: Shengkun Tong   Group: iGEM20_HZAU-China   (2020-10-17)
Revision as of 10:06, 18 October 2020 by TSK (Talk | contribs)


ClyR fused with PhoA signal peptide

ClyR is a chimeric lysine which can bind to S. mutans by a cell-wall binding domain and then lysis its cell wall from outside by a catalytic domain. ClyR has no activity for Gram-negative bacteria and can be expressed in Escherichia coli. PhoA fused ClyR can be secreted to the outside of the cells, killing S. mutans in the environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 826
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 190
    Illegal AgeI site found at 274
    Illegal AgeI site found at 589
    Illegal AgeI site found at 772
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/lysis
Parameters
None