Part:BBa_K141001

Ucp-

Apart from monitoring temperature evolution, we also characterized O.D. variations of each of our strains. We took O.D. measures every one a half hours for nine hours. We carried out this experiment both in Erlenmeyer flasks in the 30ºC shaking stove and in our LCCs. This measurements were useful in order to determine some parameters for our Black Box Model. Besides, we were able to prove that the UCP was indeed being produced even though we could not see the temperature increase. Since our mutant strains Gly175Δ and Gly76Δ do not have the same growing rate as UCP+ when they are expressing the protein, the difference between the results in the strains showed that the reason for our lack of temperature increase was that we had not found the optimum conditions yet. This results made us keep on working until we obtained successful results.

Strains growth equations:

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 573
  Illegal XbaI site found at 873
  Illegal PstI site found at 289
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 289
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 573
  Illegal XbaI site found at 873
  Illegal PstI site found at 289
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 573
  Illegal XbaI site found at 873
  Illegal PstI site found at 289
  Illegal AgeI site found at 726
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 674