Composite

Part:BBa_K3598022

Designed by: Ruijuan Xiang   Group: iGEM20_Beijing_4ELEVEN   (2020-10-23)
Revision as of 09:05, 23 October 2020 by Registry (Talk | contribs)

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LacI_LacI promoter_T7 promoter_lac operator_RBS lib_mTyr-CNK_T7 terminator

This is the RBS lib construction system for mTyr-CNK expression. The T7 promoter is regulated by lacI repressor through lacO operator to control the expression of mTyr-CNK. The system is used to produce mTyr-CNK, so we construct a RBS library to improve the yield of mTyr-CNK.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1280
    Illegal BsaI.rc site found at 1270


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Categories
Parameters
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