Part:BBa_K3431050
Banana_ToeholdSwitch-Regulated sfGFP
Introduction
The sequence of this part is from a modular synthetic biology education kit, banana toehold sensor sfGFP (Biobits) 1 . This composite part can be triggered by the RNA of banana and express sfGFP (BBa_K3431031) as its reporter.
Characterization
The 2020 iGEM CSMU_Taiwan carried out a functionality test on this part, measuring the real-time fluorescence of the sfGFP within the 80 minutes protein production process. The result is shown below.
Results As seen, the sfGFPs would be highly expressed when the banana triggers existed, while they would be inhibited when there was no trigger in the environment. This suggested the regulatory function of the toehold switch.
Reference
1. Huang, A., Nguyen, P. Q., Stark, J. C., Takahashi, M. K., Donghia, N.,Ferrante, T., Dy, A. J., Hsu, K. J., Dubner, R. S., Pardee, K., Jewett, M. C., &Collins, J. J. (2018). BioBits™ Explorer: A modular synthetic biology education kit. Science advances, 4(8), eaat5105. https://doi.org/10.1126/sciadv.aat5105
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 865
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |