Part:BBa_K3552003

hofB-hofC(gene from DH5a)

HofB is a transport protein found in bacteria and is ATP-binding and nucleotide-binding, constitute the generator of pilA. HofC is an inner membrane protein, a base for the generator of pilA. This part is in the part collection where we have 13 genes that code for the proteins.

The hofC is from the hof operon encoding the generator of major pilin, the ATPase HofB and the platform protein HofC is localized at 3 min of E.coli map. The HofB gene is a ATPase motor which localized at the base of the pilin generator system in the inner membrane, propel the assembly of pilus and it interacts with the platform protein HofC and HofM component of the inner membrane anchored assembly platform complex that also includes HofN and HofO. The hofMNOPQ operon encodes the assembly platform complex connecting HofB ATPase with the secretin hofQ.

We first uses the bacteria DH5a as the template and obtain the DNA fragment of 12 genes. We verified the practicability of the generator through the western blot of pilA in vivo which confirmed the usage of the generator because the PilA is able to display outside the cell.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 849
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1778
  
• 1000
  COMPATIBLE WITH RFC[1000]