Part:BBa_K165061

pRS304* yeast shuttle vector, TRP1 selection

This is a vector specifically designed for integration into a yeast genome at the LEU2 gene.

The final construction step of a Biobrick device can be made into this vector but cutting with the following scheme:

The system works in a yeast strain that has a mutation in the LEU2 gene, meaning that it is incapable of producing leucine on its own. Untransformed yeast cells with this mutation must be grown in media that contains leucine in order to be viable. However, after a transformation protocol in which competent yeast cells are treated with the pRS304* plasmid

Yeast integration

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1942
  Illegal XbaI site found at 550
  Illegal XbaI site found at 1912
  Illegal SpeI site found at 1918
  Illegal PstI site found at 251
  Illegal PstI site found at 1936
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1942
  Illegal SpeI site found at 1918
  Illegal PstI site found at 251
  Illegal PstI site found at 1936
  Illegal NotI site found at 1904
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1942
  Illegal BamHI site found at 1924
  Illegal XhoI site found at 1975
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1942
  Illegal XbaI site found at 550
  Illegal XbaI site found at 1912
  Illegal SpeI site found at 1918
  Illegal PstI site found at 251
  Illegal PstI site found at 1936
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1942
  Illegal XbaI site found at 550
  Illegal XbaI site found at 1912
  Illegal SpeI site found at 1918
  Illegal PstI site found at 251
  Illegal PstI site found at 1936
  Illegal NgoMIV site found at 1563
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3348
  Illegal SapI site found at 2265