Coding

Part:BBa_K3573002:Experience

Designed by: Jungwoo Choe   Group: iGEM20_Korea_HS   (2020-10-11)
Revision as of 04:20, 23 October 2020 by Jchoe21 (Talk | contribs) (→‎Applications of BBa_K3573002)


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Applications of BBa_K3573002

Korea_HS team have performed binding assays using size-exclusion chromatography. We ran three separate runs of 3 superdex75 10/300GL column with buffer (20mM Tris-HCl pH 7.0+250mM NaCl)

1. scFv(RAS) only 2. HRAS only 3. scFv(RAS)+HRAS

The result showed that HRAS's elution peak shifted to higher molecular weight position in the presence of scFv(RAS) compared to HRAS only, indicating their binding.

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