Composite

Part:BBa_K3409011

Designed by: Albane Mabro   Group: iGEM20_Ionis_Paris   (2020-10-17)
Revision as of 19:00, 17 October 2020 by Registry (Talk | contribs)

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Microcin PDI cluster gene for McpD

Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: a promoter (BBa_J23118), an RBS (BBa_J61118), the CDS for McpD (BBa_K3409006). Will constitutively generate McpD. For appropriate secretion, it must be followed by BBa_K3409012, which contains the CDS for McpB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None