Part:BBa_K3440017

bphR2 under bphR1 promoter

Pconst(BBa_J23100)-RBS(BBa_B0034)-bphR2 mutated(BBa_K1413021)-Myc(BBa_K823036)-DT(BBa_B0015)-Pbphr1(BBa_K1155001)-RBS(BBa_B0034)-mCherry(BBa_J06504)

Usage and Biology

This part can be used to prove that bphR1 can be activated by expression of bphR2. bphR2 is constitutively expressed and can induce bphR1 promoter, under which a reporter mCherry was placed. Induction can be enhanced in the presence of polychlorinated biphenyls (PCB), which can form an activating complex with bphR2

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 996
  Illegal BamHI site found at 1301
  Illegal BamHI site found at 1384
  Illegal XhoI site found at 1191
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 536
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 320
  Illegal SapI.rc site found at 1284