Part:BBa_K3440014

GFP under Plux in presence of LuxR and AHL

Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxR(BBa_C0062)-Myc(BBa_K823036)-DT(BBa_B0015)-Plux(BBa_B0015)-RBS(BBa_B0034)-GFP(BBa_E0040)

Usage and Biology

This part produces GFP in the presence of 3OC6-HSL. 3OC6-HSL can from a complex with LuxR, which is expressed constitutively. the complex can activate Plux promoter, under which GFP is placed.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.

Western blot with Q3 as BBa_K3440014

Fluorescent measurements with Q3 as BBa_K3440014

Fluorescence of BBa_K3440014 in the presence of 3OC6-HSL under UV light

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 874
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1023
  Illegal BsaI.rc site found at 1750