Measurement
Part:BBa_K079026
Designed by: Maria Vittoria Amaduzzi Group: iGEM08_Bologna (2008-10-24)
GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter
The GFP reporter protein was put under the control of the BBa_J23118 costitutive promoter. The reporter sequence was spaced from the promoter by the addition of the BBa_C0012 sequence without the RBS. This construct was design as an expression control plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1864
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Categories
Parameters
None |