Tag

Part:BBa_K3589151

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-24)
Revision as of 14:32, 24 October 2020 by Registry (Talk | contribs)

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HA-RGS-8xHis tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of an HA- as well as an 8xHis-tag (B5). Combined with a CDS, an appropriate promoter and a terminator your target protein should be detectable using an HA-antibody and you should be able to purify it using a nickel-NTA-column.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None