Tag
Part:BBa_K3589151
Designed by: Yannik Schermer Group: iGEM20_TU_Kaiserslautern (2020-10-24)
HA-RGS-8xHis tag for Chlamydomonas reinhardtii (Phytobrick)
This basic part contains the sequence of an HA- as well as an 8xHis-tag (B5). Combined with a CDS, an appropriate promoter and a terminator your target protein should be detectable using an HA-antibody and you should be able to purify it using a nickel-NTA-column.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |