Coding

Part:BBa_K3429002

Designed by: Jan Lars Kalkowski   Group: iGEM20_TU_Darmstadt   (2020-09-19)
Revision as of 15:31, 25 October 2020 by Mehryad (Talk | contribs)


Superfolder Green Fluorescent Protein (sfGFP)

Profile

Name TasA matrix protein
Base pairs 786
Molecular weight 26.8 kDa
Origin Aequorea victoria, synthetic

The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. [1] We implement it as a reporter and experimental control for our TasA fusion proteins with the enzymes CotA, CuO and EreB.


References

[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None