Composite
Part:BBa_K3504022
Designed by: Ahmed Wael Group: iGEM20_AFCM-Egypt (2020-10-25)
Alphavirus replicon NSPs- SFV
To be edited
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2766
Illegal EcoRI site found at 4344
Illegal XbaI site found at 7239
Illegal PstI site found at 1018
Illegal PstI site found at 3378
Illegal PstI site found at 4392
Illegal PstI site found at 5045
Illegal PstI site found at 5110
Illegal PstI site found at 6970 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2766
Illegal EcoRI site found at 4344
Illegal NheI site found at 1224
Illegal NheI site found at 3065
Illegal NheI site found at 6732
Illegal PstI site found at 1018
Illegal PstI site found at 3378
Illegal PstI site found at 4392
Illegal PstI site found at 5045
Illegal PstI site found at 5110
Illegal PstI site found at 6970
Illegal NotI site found at 6736 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2766
Illegal EcoRI site found at 4344
Illegal BglII site found at 7314
Illegal XhoI site found at 5897 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2766
Illegal EcoRI site found at 4344
Illegal XbaI site found at 7239
Illegal PstI site found at 1018
Illegal PstI site found at 3378
Illegal PstI site found at 4392
Illegal PstI site found at 5045
Illegal PstI site found at 5110
Illegal PstI site found at 6970 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2766
Illegal EcoRI site found at 4344
Illegal XbaI site found at 7239
Illegal PstI site found at 1018
Illegal PstI site found at 3378
Illegal PstI site found at 4392
Illegal PstI site found at 5045
Illegal PstI site found at 5110
Illegal PstI site found at 6970
Illegal NgoMIV site found at 2211
Illegal NgoMIV site found at 2448
Illegal NgoMIV site found at 3558
Illegal NgoMIV site found at 4295
Illegal NgoMIV site found at 4305
Illegal NgoMIV site found at 5971
Illegal AgeI site found at 5965 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3115
Illegal BsaI.rc site found at 4598
Illegal BsaI.rc site found at 7229
[edit]
Categories
Parameters
None |