Composite

Part:BBa_K3606059:Design

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)
Revision as of 19:39, 23 October 2020 by Registry (Talk | contribs)

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P3 driven mcbABCD and PtetR driven mcbEFG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5559
    Illegal XbaI site found at 5544
    Illegal PstI site found at 2382
    Illegal PstI site found at 2415
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5559
    Illegal PstI site found at 2382
    Illegal PstI site found at 2415
    Illegal NotI site found at 5551
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5559
    Illegal BamHI site found at 3378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5559
    Illegal XbaI site found at 5544
    Illegal PstI site found at 2382
    Illegal PstI site found at 2415
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5559
    Illegal XbaI site found at 5544
    Illegal PstI site found at 2382
    Illegal PstI site found at 2415
    Illegal NgoMIV site found at 2044
    Illegal AgeI site found at 2216
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

tba


Source

tba

References