Composite
Part:BBa_K3606059:Design
Designed by: Yuanyuan Liu Group: iGEM20_Fudan (2020-10-23)
P3 driven mcbABCD and PtetR driven mcbEFG
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5559
Illegal XbaI site found at 5544
Illegal PstI site found at 2382
Illegal PstI site found at 2415 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5559
Illegal PstI site found at 2382
Illegal PstI site found at 2415
Illegal NotI site found at 5551 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5559
Illegal BamHI site found at 3378 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5559
Illegal XbaI site found at 5544
Illegal PstI site found at 2382
Illegal PstI site found at 2415 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5559
Illegal XbaI site found at 5544
Illegal PstI site found at 2382
Illegal PstI site found at 2415
Illegal NgoMIV site found at 2044
Illegal AgeI site found at 2216 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
tba
Source
tba