![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3606068:Design
Designed by: Yuanyuan Liu Group: iGEM20_Fudan (2020-10-23)
P12 driven mcbABCD and PtetR driven mcbEFG
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4794
Illegal PstI site found at 2297
Illegal PstI site found at 2330 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4794
Illegal PstI site found at 2297
Illegal PstI site found at 2330 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4794
Illegal BglII site found at 2204 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4794
Illegal PstI site found at 2297
Illegal PstI site found at 2330 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4794
Illegal PstI site found at 2297
Illegal PstI site found at 2330
Illegal NgoMIV site found at 1959
Illegal AgeI site found at 2131 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5055
Design Notes
McbABCD and McbEFG are placed in reverse, and each is independently expressed under different promoters with their own terminator.
Source
McbABCD and McbEFG orginally come from a plasmid of Escherichia coli.