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Coding
Part:BBa_K3468044:Experience
Designed by: Yufei Zhou Group: iGEM20_TJUSLS_China (2020-10-19)
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Applications of BBa_K3468044
According to the prediction of FoldX, DDG decreased, and PyOML was used for visual screening, which formed hydrogen bonds with 132-ARG, 139-GLY and 140-THR. after mutating it into ASN, the number of hydrogen bonds increased, which stabilized α3-β5loop and was beneficial to improve the thermal stability of PETase.
File:S136R.png
Fig.1 S136R in PyMOL
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