Composite
Part:BBa_K3606031:Design
Designed by: Zhenru Zhang,Yiyang Lu Group: iGEM20_Fudan (2020-10-23)
Nsp4-CaAP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
Illegal AgeI site found at 135 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We linked NSP4 to the N-terminal of CAAP for its secretion into the extracellular space.
Source
The sequences of subparts are obtained from E.coli. genome.