Composite
Part:BBa_K3332076
Designed by: Meihao Ou Group: iGEM20_XMU-China (2020-10-17)
J23101-OmpA 5'UTR-phnJ 0.94-Hfq binding sequence-J61048
Encoding the siRNA squence which can silence the phnJ gene in E.coli BL21(DE3).Use K823005 to silence the phnJ gene in E.coli BL21 (DE3).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 281
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 294
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |