Composite

Part:BBa_K3332069

Designed by: Siru Zhou   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 15:12, 17 October 2020 by Registry (Talk | contribs)

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J23100-RBS-phnJ_mut21-terminator

Use BBa_K823004-B0034 to express the mutant of C-P lyase, which can degrade the glyphosate, from Enterobacterales. This subunit is 

essential to the activity of the whole enzyme.Improve the capability of chassis bacteria to degrade glyphosate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 610
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 658
    Illegal AgeI site found at 329
    Illegal AgeI site found at 836
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None