Composite

Part:BBa_K3332099

Designed by: Shiyi Zhai   Group: iGEM20_XMU-China   (2020-10-18)
Revision as of 18:48, 24 October 2020 by RuiJie Mo (Talk | contribs)


RNAi sequence of phnF(0.97)-RNAi sequence of phnJ(0.69)

Encoding the siRNA squence which can silence the phnF and phnJ gene in E.coli BL21(DE3).Use K823005 to silence the phnF gene in E.coli BL21 (DE3).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 438
    Illegal NheI site found at 461
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 281
    Illegal XhoI site found at 712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None