Composite

Part:BBa_K3490031:Design

Designed by: Ren Hao Tsai   Group: iGEM20_NCKU_Tainan   (2020-10-23)
Revision as of 14:38, 23 October 2020 by Registry (Talk | contribs)

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The toxin-antitoxin system: hicA-hicB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 109
    Illegal BsaI.rc site found at 149


Design Notes

hicA and hicB need to locate in different copy number plasmid to mediate the expression of hicA and hicB. hicA is located in pSB1C3 with pBad promoter while hicB is located in pSB3K3 with J23100.



Source

Derived from IDT synthesis.

References