Composite
Part:BBa_K3490021:Design
Designed by: Yao Tien Cheng Group: iGEM20_NCKU_Tainan (2020-10-23)
Verify the function of EL222-pBlind and FLP-FRT systems.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1573
Illegal SpeI site found at 1891 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1573
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 3035
Illegal NheI site found at 3058
Illegal SpeI site found at 1891 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1573
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1573
Illegal SpeI site found at 1891 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1573
Illegal SpeI site found at 1891
Illegal NgoMIV site found at 142
Illegal AgeI site found at 367 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2882
Illegal BsaI.rc site found at 3115
Design Notes
during the experiment process, we had difficulty constructing both plasmids. With the doubt that the EL222-FLP system might be harmful to the growth of bacteria, we changed the design of blue-sensitive protein EL222 into thermo-sensitive protein CI857 on the plasmid pCP20 provided by our PI, Prof. Ng
Source
Derived from IDT synthesis.