Signalling

Part:BBa_K3697008

Designed by: Lauren Ramlan   Group: iGEM20_Stanford   (2020-10-23)
Revision as of 08:17, 23 October 2020 by Registry (Talk | contribs)

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B. subtilis YFP CDS

This part is a yellow fluorescent protein optimized for use in Bacillus subtilis. It can be used as a reporter or signaling protein in B. subtilis or E. coli. Although it is optimized for expression in B. subtilis, E. coli are capable of translating this YFP. When highly expressed in B. subtilis, it will produce a signal that can be visualized using a plate reader. When highly expressed in E. coli, it produces a signal that can be seen with the naked eye. YFP has and excitation peak of 514 nm and emission peak of 527 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
None