Signalling

Part:BBa_K3365006:Design

Designed by: JUNYAN LI   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-23)
Revision as of 03:34, 25 October 2020 by Little ghost (Talk | contribs) (→‎Design Notes)


Target sequence downstream of pBAD/araC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The target sequence is from the literature and ligated to the inducible pBAD/araC promoter by PCR.

Source

PCR

References