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Part:BBa_K3573001:Experience

Designed by: Jungwoo Choe   Group: iGEM20_Korea_HS   (2020-10-10)
Revision as of 02:54, 25 October 2020 by Jchoe21 (Talk | contribs) (→‎Applications of BBa_K3573001)


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Applications of BBa_K3573001

Our team has cloned scFv(RAS) into an expression vector and tested the protein expression in 4 different conditions (2 temperatures X 2 IPTG concentrations)

After expression, we used Ni-NTA column for protein purification using the His6-tag located at the N-terminus of CPP-scFv(RAS). We have confirmed the identity of the protein using Western blot analysis using anti-His6 antibody.

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