Composite

Part:BBa_K3560005

Designed by: ShiDi Xiao   Group: iGEM20_XHD-Wuhan-China   (2020-10-23)
Revision as of 09:21, 23 October 2020 by Registry (Talk | contribs)

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PGroES-DrRBS-gabY

gabY promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, and enhances its catalytic efficiency. The gabY fragment was recombined with pRADK to form gabY-pRADK, Transform gabY-pRADK into DR containing gcd-pRADK plasmids, so that DR containing gabY-pRADK and gcd-pRADK plasmids can decrease the pH to a greater extent and promote the dissolution of phosphate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 399
    Illegal NgoMIV site found at 565
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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