Part:BBa_K3493000:Design
G. algens dextranase CDS
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 385
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 385
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 385
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 385
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization for E. coli. Following reports that the Streptococcus mutans homologous dextranase has a higher activity and resistance to proteolysis when its N terminal (residues 1-99) and C-terminal (residues 733-850) are deleted (Kim et al. 2011. Appl. Microbiol. Biotechnol.), we removed them from the CDS submitted to the registry. Accordingly, our final CDS has the contains the region of the G. algens dextranase sequence that aligns to residues 104 - 734 of the PDB structure of the S. mutans dextranase (Suzuki, et al. 2012. J. Biol. Chem.).
Source
The protein sequence is available at NCBI (https://www.ncbi.nlm.nih.gov/protein/WP_066432599.1?report=genbank&log$=prottop&blast_rank=700&RID=MZBBN4NZ014). The gene sequence was inferred from the protein sequence using codon optimization for E. coli.