Plasmid

Part:BBa_K3505011

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-20)
Revision as of 13:19, 24 October 2020 by Venetios (Talk | contribs)


pDGB1 omega 2 (Ω2) for Bacterial GoldenBraid

Level Ω vector for Golden Braid Cloning. Having cargo LacZa for blue white screening between BsaI and BsmBI sites.Resistance in Spectinomycin and ori pBBR1 for multiple protein expression capacities.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3277
    Illegal XbaI site found at 3304
    Illegal PstI site found at 3316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3277
    Illegal PstI site found at 3316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3277
    Illegal BamHI site found at 3298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3277
    Illegal XbaI site found at 3304
    Illegal PstI site found at 3316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3277
    Illegal XbaI site found at 3304
    Illegal PstI site found at 3316
    Illegal NgoMIV site found at 948
    Illegal NgoMIV site found at 1350
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2918
    Illegal BsaI.rc site found at 6
[edit]
Categories
//awards/part_collection
//plasmid/construction
//plasmid/expression
Parameters
None