Coding

Part:BBa_K3373002:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-13)
Revision as of 21:00, 13 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


atzB P. sp. ADP codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 1100
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CDS is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends.

The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.


Source

Pseudomonas sp. (strain ADP) 47660 [NCBI]

References