Composite

Part:BBa_K3490001

Designed by: Ryan Huang   Group: iGEM20_NCKU_Tainan   (2020-10-23)
Revision as of 13:59, 23 October 2020 by Registry (Talk | contribs)

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IPTG inducible NOS, over-express csgD and csgA

During literature research, we found out that Bacillus subtilis carries Nitric Oxide Synthases and has the ability to produce NO. So we cloned Bacillus subtilis' NOS gene under the control of an inducible promoter, pLacI, into a plasmid and transformed it into E. coli. In order to induce our target gene, NOS, with IPTG, we put a LacI and T7 promoter in front of NOS and LacO after it. We chose WM3064 as our bacteria strain because it contains T7 RNA polymerase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3923
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1826
    Illegal SapI.rc site found at 2144


[edit]
Categories
//awards/composite_part/nominee
Parameters
None