Coding
Part:BBa_K3381002:Design
Designed by: Jeriann Hsiao Group: iGEM20_Waterloo (2020-10-20)
Mst-CopC (copper-binding)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for E. coli, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.
Source
Methylosinus trichosporium O3B, sequence found through GenBank https://www.ncbi.nlm.nih.gov/protein/ATQ66671
References
Lawton, T. J., Kenney, G. E., Hurley, J. D., & Rosenzweig, A. C. (2016). The CopC Family: Structural and Bioinformatic Insights into a Diverse Group of Periplasmic Copper Binding Proteins. Biochemistry, 55(15), 2278-2290. doi:10.1021/acs.biochem.6b00175.s003