Protein_Domain

Part:BBa_K3373009:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-23)
Revision as of 23:15, 23 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


N-terminal region of TEV protease optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 228
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 228
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CDS is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B5 (C-terminus coding region) position by adding BbsI sites and the corresponding fusion sites on both ends. The stop codon was added after the NLS sequence.


Source

Tobacco etch virus (TEV) 12227 [NCBI]

References