Coding

Part:BBa_K3373008:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-23)
Revision as of 22:32, 23 October 2020 by Registry (Talk | contribs)

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NucleaseA fused to the nuclear localization signal (NLS) of SV40 codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CDS is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B5 (C-terminus coding region) position by adding BbsI sites and the corresponding fusion sites on both ends. The stop codon was added after the NLS sequence.


Source

Staphylococcus aureus 1280 [NCBI]

References