Composite

Part:BBa_K3338023

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)
Revision as of 22:09, 23 October 2020 by Registry (Talk | contribs)

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IL-6 Pmut-MagA-P2A-hGLuc

IL-6 P-MagA-P2A-hGLuc is an inflammatory toxin sensor comprising the IL6-Promoter downstream from the MagA-P2A-hGLuc cassette. This part combines the LPS-sensitivity of the IL6-Promoter with the simultaneous expression of the reporter genes MagA for MRI-detection and Gaussia Luciferase for bioluminescence detection in blood or urine (Goldhawk et al. 2009, Zurkiya et al. 2008, Tannous 2009). The construct displays the first step in generating a functional inflammatory toxin sensor for clinical use.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
    Illegal BamHI site found at 395
    Illegal XhoI site found at 1581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 340
    Illegal BsaI site found at 2803
    Illegal BsaI.rc site found at 387
    Illegal BsaI.rc site found at 696
    Illegal BsaI.rc site found at 2135
    Illegal BsaI.rc site found at 2684
    Illegal SapI site found at 1825


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Categories
Parameters
None