Coding
Part:BBa_K3582026:Design
Designed by: Saillesh Aravindhan C Group: iGEM20_IISER-Pune-India (2020-10-20)
Inhibitory sequence 1 of PfEMP1 - ICAM1 interaction grafted into a cyclotide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 112
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 143
Illegal BsaI.rc site found at 109
Design Notes
- 1] RFC10 criteria satisfied
- 2] Strep Ⅱ tag gene inserted in loop 3
- 3] Codon optimized with E-coli K-12 strain
- 4] Inhibitor 1 grafted in loop 6
- 5] C and N intein added for circularization
Source
Reverse translation of amino acid sequence
References
- 1] Gould, A., Ji, Y., Aboye, T. L., & Camarero, J. A. (2011, December). Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330703/
- 2] DJ;, P. A. (n.d.). Cyclotides as grafting frameworks for protein engineering and drug design applications. Retrieved from https://pubmed.ncbi.nlm.nih.gov/23893608/
- 3] Camarero, J. A., & Campbell, M. J. (2019, April 19). The Potential of the Cyclotide Scaffold for Drug Development. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631875/