Regulatory

Part:BBa_K3338005:Design

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-22)
Revision as of 20:29, 22 October 2020 by Registry (Talk | contribs)

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Interleukin-6 Promoter mutagenized (IL-6 Pmut)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
    Illegal BamHI site found at 395
    Illegal XhoI site found at 1581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 340
    Illegal BsaI.rc site found at 387
    Illegal BsaI.rc site found at 696


Design Notes

The original sequence of the IL6P was modified so that it is compatible with the RFC10 assembly standard. Therefore, a point mutation in a XbaI site was introduced. There is no predicted clash with a NF-κB or an AP1 transcription factor binding site.


Source

The sequence was synthesized by IDT.

References